產(chǎn)品名稱：人抗利尿激素（ADH）

英文名稱：  Human antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA KIT                                                             

產(chǎn)品規(guī)格：96T/48T                                                                      

品牌：RD/RB                                                                  

類別：ELISA試劑盒/人的ELISA檢測試劑盒                                             

上海恒遠(yuǎn)生物公司全部備有現(xiàn)貨，有需要的請及時(shí)垂詢：,

“ 人抗利尿激素（ADH）”操作步驟（中文）                                                                 

1.標(biāo)準(zhǔn)品的稀釋：本試劑盒提供原倍標(biāo)準(zhǔn)品一支，用戶可按照下列圖表在小試管中進(jìn)行稀釋。                                                                   

8 ng/L    5號標(biāo)準(zhǔn)品  150μl的原倍標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液                                                   

4 ng/L    4號標(biāo)準(zhǔn)品  150μl的5號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液                                                   

2 ng/L    3號標(biāo)準(zhǔn)品  150μl的4號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液                                                   

1 ng/L    2號標(biāo)準(zhǔn)品  150μl的3號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液                                                   

0.5 ng/L 1號標(biāo)準(zhǔn)品  150μl的2號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液                              

2.加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑，其余各步操作相同）、標(biāo)準(zhǔn)孔、                                             

待測樣品孔。在酶標(biāo)包被板上標(biāo)準(zhǔn)品準(zhǔn)確加樣50μl，待測樣品孔中先加樣品稀釋液40μl，然后                                                

再加待測樣品10μl（樣品zui終稀釋度為5倍）。加樣將樣品加于酶標(biāo)板孔底部，盡量不觸                                              

及孔壁，輕輕晃動混勻。                                              

3.溫育：用封板膜封板后置37℃溫育30分鐘。                                               

4.配液：將20倍濃縮洗滌液用蒸餾水20倍稀釋后備用                              

5.洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復(fù)                                                

5次，拍干。                                              

6. 加酶：每孔加入酶標(biāo)試劑50μl，空白孔除外。                                              

7.溫育：操作同3。                                          

8.洗滌：操作同5。                                          

9.顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘.                                            

10.終止：每孔加終止液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。                   

11.測定：以空白空調(diào)零，450nm波長依序測量各孔的吸光度（OD值）。 測定應(yīng)在加終止液后                                             

15分鐘以內(nèi)進(jìn)行。                                           

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